Flow Cytometry ：(FCM) is a powerful tool for multi-parameter analysis or sorting of cells in heterogeneous cell populations. Under the condition that the structure and function of cells and organelles are not destroyed, various signals can be obtained from the molecular level for quantitative analysis or purification and sorting of cells, and the measurement is fast, large, accurate, sensitive and quantitative.
It can be used for a series of applications such as immunophenotyping (white blood cell), ploidy analysis (DNA), cell counting, and GFP (green fluorescent protein) expression analysis. An analytical technique that detects multiple physical, chemical and functional indicators of a single cell or particle in a flowing state.
Features of Flow Cytometry: Strong specificity, high sensitivity, fast speed, and can realize multi-parameter analysis.
The principle of flow detection: Excite various fluoresceins and fluorescent dyes carried by a single cell flowing at a high speed by laser, and detect the resulting scattered light and fluorescent emission light, and reflect the cell's performance through the intensity of various light signals. Various characteristics.
Main components of Flow Cytometry: Liquid flow system, optical path system, signal measurement and cell sorting, etc.
Performance of Flow Cytometry lasers:
• High power stability, low noise
• Good pointing stability
• Single-laser module/ Multi-laser platform optional
• CE certificated
Commonly used wavelengths:
405nm, 473nm, 488nm, 532nm, 561nm, 593.5nm, 638nm, 671nm, 785nm, etc.